Nestin Antibody orb334607 from Biorbyt

Product Specs
ItemNestin Antibody
CompanyBiorbyt
Price Pricing Info Supplier Page
Catalog Numberorb334607
Quantity10 µg, 100 µg
ApplicationsIHC, WB
Conjugate/TagUnconjugated
FormatLyophilized
ReactivityHuman, Mouse, Rat
TargetNestin
IsotypeRabbit IgG
HostRabbit
ImmunogenA synthetic peptide corresponding to a sequence at the N-terminus of human Nestin (252-290aa ERLRATEKFQLAVEALEQEKQGLQSQIAQVLEGRQQLAH), different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids
NCBI Full Gene Namenestin
NCBI Gene AliasesNbla00170
ClonalityPolyclonal
Molecular Weight177439 MW
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 µg/ml
StorageStore at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles

Specifications/Features

Application Notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Application Dilutions: Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, By HeatImmunohistochemistry (Frozen Section), 0.5-1µg/ml Western blot, 0.1-0.5µg/ml

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